Journal: Redox Biology

Article Title: Divergent redox responses of macular and peripheral Müller Glia: Implications for retinal vulnerability

doi: 10.1016/j.redox.2025.103691

Figure Lengend Snippet: Knockdown of MT1G , AKAP12 and MAFF in human primary Müller glia. A. MT1G , AKAP12 and MAFF siRNA knockdown in primary Müller glia exposed to light stress. B & C . AlamarBlue cell viability assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA treatment in response to light stress. n = 6 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. Data are presented as means ± standard error of the mean (SEM). D & E . JC1 assay on primary Müller glia with or without MT1G , AKAP12 and MAFF siRNA knockdown in response to light stress. n = 8 biological replicates per group. Statistical analysis was performed using Welch's t -test (two-sided) between the control group (used consistently across all pairwise comparisons) and the respective knockdown groups. F – H . Volcano plots of differential gene expression (p < 0.05, FC > 1.5) in MT1G , AKAP12 and MAFF siRNA knockdown vs. control groups. I – K . IPA of differential gene expression in human primary Müller glia with MT1G , AKAP12 and MAFF siRNA knockdown compared to the control group in response to light stress.

Article Snippet: Human primary Müller glia (P3) were transfected with AKAP12 , MT1G , MAFF and control siRNA in a 96-well plate for three days, then starved overnight and exposed to light stress for 4 h. Cells were treated with AlamarBlue reagent (1:10 dilution, #DAL1100, ThermoFisher Scientific) and incubated at 37 °C for 4 h using the AlamarBlue cell viability assay kit.

Techniques: Knockdown, Viability Assay, Control, Gene Expression

Journal: bioRxiv

Article Title: Ribosomal protein L5 (RPL5/uL18) I60V mutation is associated to increased translation and modulates drug sensitivity in T-cell acute lymphoblastic leukemia cells

doi: 10.1101/2025.07.16.665036

Figure Lengend Snippet: The effect of the drugs on cellular metabolism was assessed by treating RPL5-WT (blue) and RPL5-I60V (red) clones with various anticancer drugs, primarily targeting translational machinery. Metabolic activity was evaluated, after a 48-hour treatment period, by AlamarBlue ™ assay and data presented as fold change vs not treated sample. Graphs represent the average of two experiments run in duplicate. MNK1: MNK1 inhibitor; MTF: metformin; SIL: silvestrol; HHT: homoharringtonine; ANI: anisomycin; RV: resveratrol; HYG B: hygromycin B; ARA-C: cytarabine. Statistical significance was assessed by two-way ANOVA with Sidak’s multiple comparisons test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Alamarblue TM reagent (ThermoFisher Scientific) was added directly into the culture media at a final concentration of 10%, and the plate was incubated for 4 hours at 37 °C, 5% CO 2 , after which fluorescence was measured in a Spark plate reader (TECAN).

Techniques: Clone Assay, Activity Assay, Alamar Blue Assay